Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: A Topical Chinese Herbal Alleviates Psoriasis by Regulating Keratinocytes Pyroptosis Through Inhibition of NLRP3 Inflammasome Activation

doi: 10.2147/CCID.S559712

Figure Lengend Snippet: ( A – C ) mRNA levels of NLRP3, Caspase-1, GSDMD in NHEK cells, n = 3. ( D – I ) Protein levels of NF-κB, P65, NLRP3, caspase-1, GSDMD in NHEK cells, n = 3.*P < 0.05, **P < 0.01, ***P < 0.001 compared with Control; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with Model; & P < 0.05, && P < 0.01, &&& P < 0.001 compared with QS; ^P < 0.05, ^^P < 0.01 compared with Mcc950.

Article Snippet: The sections were then incubated overnight at 4°C with the following primary antibodies: NF-κB p65 Monoclonal antibody (66535-1-Ig, Proteintech, China), NLRP3 Monoclonal antibody (68102-1-Ig-100, Proteintech, China), Caspase-1 antibody (sc-56036, Santa Cruz, USA), and GSDMD Polyclonal antibody (20770-1-AP, Proteintech, China).

Techniques: Control

Journal: Life

Article Title: Persicaria minor (Huds.) Opiz Prevents In Vitro Atherogenesis by Attenuating Tumor Necrosis Factor-α-Induced Monocyte Adhesion to Human Umbilical Vein Endothelial Cells

doi: 10.3390/life12101462

Figure Lengend Snippet: Nuclear factor kappaB (NF-κB) p65 levels in the nuclear extract of human umbilical vein endothelial cells (HUVEC). Values are expressed as mean ± SEM, n = 7 (* p < 0.05 vs. control. # p < 0.05; ### p < 0.001 vs. TNF-α). PM, standardized aqueous extract of P. minor leaves; Q3G, quercetin-3- O -glucuronide; TNF-α, tumor necrosis factor-α.

Article Snippet: Human NF-κB p65 subunit ELISA kit (Elabscience, Houston, TX, USA) was utilized to determine NF-κB p65 concentration in the nuclear extract of HUVEC.

Techniques: Control

Journal: Molecules

Article Title: Application of Cinnamomum burmannii Essential Oil in Promoting Wound Healing

doi: 10.3390/molecules29092080

Figure Lengend Snippet: BEO attenuated the inflammatory response in RAW 264.7 cells stimulated by LPS. ( A ) qPCR of IL-1β, IL-6 and TNF-α levels in RAW 264.7 cells that were stimulated by LPS at 24 h from different groups. ( B ) The level of IL-6 and TNF-α secreted by RAW 264.7 cells treated with BEO was determined by ELISA. ( C ) Effects of 150 μg/mL, 200 μg/mL, 250 μg/mL and 300 μg/mL BEO on the expression of NF-κB/p-IκBα protein. ( D ) The cell typing of RAW 264.7 was detected by flow cytometry. Polarization rate ( D ) and sectional images ( E , F ) of each group are shown. n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.001; **** p < 0.0001; ns, not significant.

Article Snippet: Monoclonal antibodies against β-actin, and NF-κB p65 were purchased from MedChemExpress Inc. (MCE, Monmouth Junction, NJ, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry