Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: A Topical Chinese Herbal Alleviates Psoriasis by Regulating Keratinocytes Pyroptosis Through Inhibition of NLRP3 Inflammasome Activation

doi: 10.2147/CCID.S559712

Figure Lengend Snippet: ( A – C ) mRNA levels of NLRP3, Caspase-1, GSDMD in NHEK cells, n = 3. ( D – I ) Protein levels of NF-κB, P65, NLRP3, caspase-1, GSDMD in NHEK cells, n = 3.*P < 0.05, **P < 0.01, ***P < 0.001 compared with Control; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with Model; & P < 0.05, && P < 0.01, &&& P < 0.001 compared with QS; ^P < 0.05, ^^P < 0.01 compared with Mcc950.

Article Snippet: The sections were then incubated overnight at 4°C with the following primary antibodies: NF-κB p65 Monoclonal antibody (66535-1-Ig, Proteintech, China), NLRP3 Monoclonal antibody (68102-1-Ig-100, Proteintech, China), Caspase-1 antibody (sc-56036, Santa Cruz, USA), and GSDMD Polyclonal antibody (20770-1-AP, Proteintech, China).

Techniques: Control

Journal: Scientific Reports

Article Title: siRNA-mediated inhibition of NTT-MMP-2 reduces oxidative stress and apoptotic signaling in an ex vivo model of ischemia/reperfusion injury

doi: 10.1038/s41598-025-32875-1

Figure Lengend Snippet: Correlation of mitochondrial NTT-MMP-2 and NF-κB concentration in cytoplasm ( A ); Cytoplasmic concentration of NF-κB ( B ); Cytochrome c level in mitochondrial ( C ) and cytoplasmic ( D ) fractions. Correlation of cytochorme c in mitochondrial fraction and NF-κB ( E ), LDH as a marker of injury ( F ), HR ( G ) and RPP ( H ).

Article Snippet: NF-κB concentration in cytoplasmic fractions was measured using the Rat Nuclear Factor Kappa B (NF-κB) ELISA Kit (Cusabio, Houston, Texas, USA) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Marker

Journal: Scientific Reports

Article Title: siRNA-mediated inhibition of NTT-MMP-2 reduces oxidative stress and apoptotic signaling in an ex vivo model of ischemia/reperfusion injury

doi: 10.1038/s41598-025-32875-1

Figure Lengend Snippet: BAX ( A ) and BCL2 ( C ) gene expression in rat hearts. Correlation of BAX ( B ) and BCL2 ( D ) genes expression and NF-κB concentration in cytoplasm.

Article Snippet: NF-κB concentration in cytoplasmic fractions was measured using the Rat Nuclear Factor Kappa B (NF-κB) ELISA Kit (Cusabio, Houston, Texas, USA) according to the manufacturer’s instructions.

Techniques: Gene Expression, Expressing, Concentration Assay

Journal: bioRxiv

Article Title: Discovery and Preclinical Validation of a Clinically Optimized Mitochondrial Complex I Modulator for Alzheimer’s Disease

doi: 10.64898/2026.04.10.717554

Figure Lengend Snippet: a C273 increases resistance to oxidative stress induced by H 2 O 2 in MC65 Tet-Off cells expressing Aβ. Cells were pretreated with C273 prior to H 2 O 2 exposure, and cell viability was assessed to determine LD 50 values. LD 50 values were calculated by nonlinear regression using GraphPad Prism. b, c C273 restores intracellular NADPH ( b ) and GSH ( c ) levels in MC65 Tet-Off cells. d C273 suppresses IL-1β-induced NF-κB activation. NF-κB reporter HEK293 cells were pretreated for 24 h with C273 (50 nM, 500 nM, or 5 μM), AICAR (0.5 mM), or metformin (0.5 mM), followed by stimulation with IL-1β (1 ng/ml) for 6 h. NF-κB luciferase activity was measured using the Luciferase Assay System (Promega, E1501). n = 4 independent experiments. e C273 restores mitochondrial biogenesis, expression of ETC complexes, autophagy markers, and antioxidant proteins in MC65 Tet-Off cells. MC65 cells were cultured under Tet-On (Aβ-) or Tet-Off (Aβ+) conditions and treated with vehicle or C273 (5 nM, 50 nM, or 500 nM) for 24 h. Protein levels were analyzed by Western blot. Statistical analysis was performed via one-way ANOVA to compare the vehicle-, metformin-, AICAR- and C273-treated groups. Data are presented as mean ± SD. * P < 0.05; ** P < 0.01.

Article Snippet: NF-κB Reporter (Luc) HEK293 cell line and the antioxidant response element (ARE) Luciferase Reporter HepG2 hepatic cell line were purchased from BPS Bioscience (CA, USA) and were cultured in accordance of manufacturer instructions.

Techniques: Expressing, Activation Assay, Luciferase, Activity Assay, Cell Culture, Western Blot